NN-(4-pentynoyl)-glucosamine tetraacylated (Ac4GlcNAl) provides the first part of a simple and robust two-step technique that helps identify and characterize cell surface sialic acid-containing glycoproteins. The alkyne-modified protein is detected with either fluorescent azides or biotin azide. The acetyl groups increase cell permeability and allow the unnatural sugars to easily pass through the cell membrane. Carboxyesterases remove the acetyl groups once the monosaccharide is in the cell.

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Click Chemistry Tools offers chemical and consulting services mainly in the field of conjugation chemistry. Our experience in the field of conjugation chemistry combined with availability of many click chemistry building blocks at our facility allows us to initiate projects rapidly and to cover a broad range of conjugation chemistry related services.