Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (K_d = 10¹¹ vs. 10¹⁵M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.

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Click Chemistry Tools offers chemical and consulting services mainly in the field of conjugation chemistry. Our experience in the field of conjugation chemistry combined with availability of many click chemistry building blocks at our facility allows us to initiate projects rapidly and to cover a broad range of conjugation chemistry related services.